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Potency test kit for magic mushrooms
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Psilocybin QTest – The world’s first and only potency test kit for accurately measuring Psilocybin contents from magic mushrooms

Curious about the strength of your magic mushrooms? Tired of unpredictable trip experiences? The potency of psilocybin-containing mushrooms can vary tremendously between species, varieties, and batches. This is exactly why we developed the Psilocybin-QTest, the world’s first scientifically validated do-it-yourself test kit for measuring psilocybin concentrations.


With this test, you can easily determine the potency of any batch of dried fungal material in minutes. Results are given in both the percentage and as precise milligram amount of Psilocybin. Simply crush a large amount of mushrooms and test a small sample to know the exact psilocybin concentration of the whole batch. You can easily read the result by comparing your sample with the supplied color scale. The scale ranges from 0 to 2.5 % content, so it practically covers all natural psilocybe mushrooms. If you have even stronger mushrooms, just take half of the sample material and double the result.



Key Features of Our Test Kit:

  • Rapidly know how strong your substance is
  • Minimal sample required, user-friendly testing
  • Grind up a whole batch just test a small sample
  • University-validated method
  • The only quantitative psilocybin test on the market



Psilocybin-QTest Tutorial Video

QTest Evaluation Video – How to evaluate a QTest

Delivered components, what’s inside of the box:

Per box:

  • Pair of protective gloves
  • Detailed instructions in English
  • Evaluation scale with quick-reference guide

Per test:

  • Glass vessel with detection solution
  • Plastic vessel with extraction solution
  • 1 ml syringe
  • Sterifilt (filter for extraction)
  • Blunt cannula


If you want to learn more about our tests, you can find the respective instructions and validation according to pharmaceutical standards as well as customer reviews and frequently asked questions in the menu. If you are interested in larger bulk quantities for testing on a regular basis or would like to include our products in your portfolio as a retailer or distributor simply register in the wholesale area. If you are convinced by our tools and want to spread the word about the QTests in your community, become part of our affiliate program.


Our tests are shipped directly from the US and we offer free shipping for orders over $100 in the US and $150 in Canada. To learn more about our Shipping & Return Policy click hereIf you have any questions about the product, feel free to write to us in the contact form or send us a message on Signal at +1 (509) 870-7678 !






“We are a small, organic, and independent university startup without large venture capital funding. With your order at our webshop, you directly finance our research on new test kits like the Fentanyl QTest and make a direct contribution to harm reduction!   Our mission is to give everyone the opportunity to make self-determined decisions with the help of scientifically validated and reliable test kits.


Our QTests have also been used in the first-ever state-funded mobile drug-checking pilot project in Germany for more than 3 years!  The release of our community research project will follow shortly, which will allow you to share your experiences with us and be part of future science.”

Psilocybin-QTest Instruction Manual


For a quick overview of how to perform a QTest see the Quick Reference Guide (picture above).

Please find the most recent version of the detailed instructions below. A paper version of these instructions is always included with your test kit.



Frequently Asked Questions about the Psilocybin-QTest


Do I need anything else to perform the test?

Everything you need is included in the box.

To prepare your sample, you only need the following, which you probably already have at home:


  • A grinder or scissor for grinding & homogenizing the sample
  • A milligram scale to weigh 150 mg for the test
  • A small funnel or a creased piece of paper to pour the mushroom material into the extraction vial
  • A pot to boil water and a pair of kitchen tongs to incubate the color reaction and safely remove the detection vial for evaluation


How much material do I need for the test?

You only need 150 mg (0.15 g) of your sample to perform the test. It is best to grind down a larger batch, mix it well, and then test only 150 mg of this homogenized batch. The result will tell you the potency for the remaining material.


Does the QTest also test for Psilocin?

The Psilo-QTest quantifies the total alkaloid content (psilocybin + psilocin) and reports the summed values as a total psilocybin concentration.


Does the Psilo-QTest also work for extracts? If I want to know the potency of the extract and not the mushrooms it was made from, do I put 150 mg of the extract?

In addition to the application on dried fungal material, the rapid test shows equally reliable results for most solvent-free extracts. 

Extracts are usually more concentrated. To ensure that the color result of the test still fits the scale on the evaluation card, you have to reduce the amount of starting material accordingly. Before testing homogenize your substance. Typically, methanol extracts  are triple-concentrated, so if you have a crude extract, we recommend dividing the amount you use for the test by the factor three (50 mg). If the extract is considerably more concentrated, reduce the initial quantity accordingly. The extract must be as solvent-free as possible, as this can interfere with the extraction solution. Remember that you have to multiply the result of the color scale in the end with the used factor to get the actual result for the remaining sample. For example, weigh 50 mg (0.05 g) of material for a 3X extract. Weigh 15 mg (0.015 g) of material for a 10X extract.


What does quantitative test mean and how does the QTest differ from “classic” reagent tests?

Our QTests are more powerful than classic reagent tests. While reagent tests detect the presence of an active ingredient in a substance, our QTests quantify it. This means they determine the concentration of active ingredients in various psychoactive substances, such as magic mushrooms or magic truffles.


What is the shelf life of the QTest, and how should I store it?

Store the reagents in the refrigerator (35 - 46 °F) and out of direct sunlight for a shelf life of at least 18 months. The extraction liquid may turn slightly yellow over time. That’s okay. It is still usable.


How can I prevent the filter from clogging?

We recommend ensuring that floating fungi are not sucked into the system and positioning the sterile filter below the floating fungus layer before applying vacuum. If the process fails initially, cleaning the filter very carefully and reapplying the syringe can help. The test can still function if fungal components are transferred, but this may complicate color evaluation. Link to our video.


Do solvents interfere with the measurement?

The tests work well with, for example, rotated or evaporated ("dry") extracts, but also some solvents like ethanol work if the concentration of alkaloids is not too low. This means that if too many foreign solvents are added to your extraction, it will disturb your extraction system. The best way would be to evaporate the ethanol or any other solvent from a defined amount, note the dry weight, and measure it with the test.



Haven't found the answer you’re looking for?

Click here to find more FAQs or contact us  via or on Signal at +1 (509) 870-7678


Verification & Testimonials of the PSILOCYBIN QTEST


Compliant with established pharmaceutical testing criteria - Pharmaceutical Validation ICH Q2(R1)

Our QTests were developed by our scientist, Dr. Felix Blei, in the laboratories of the University of Jena in Germany. It is a voluntarily validated and standardized test procedure based on the pharmaceutical method validation guideline ICH Q2(R1), which we have extended to the QTests. This guideline describes the requirements of the analytical method for active ingredients that are subsequently to be used in human medicine and guarantees a safe dosage of these active ingredients, for example in pharmacies.

The PSILO-QTest was developed at the Friedrich Schiller University Jena at the Institute of Pharmacy, Chair of Pharmaceutical Microbiology, under grant number 03EGSTH1189, supported by the German Federal Ministry of Economics and Climate Protection and the European Social Fund, in the course of the third-party funded project "Production of quantitative test systems of psychotropic agents" in the period from 2019 to 2021 under the project management of Dr. Felix Blei. The necessary permission for the handling and acquisition of the narcotics psilocybin/psilocin according to § 3 of the German Narcotics Act was granted for the Friedrich Schiller University Jena under the current BtM number 463 23 75 as well as the previous one for the premises of the Pharmaceutical Microbiology in Jena. The pure substances required for the project were purchased from LIPOMED GmbH and LGC Standards Ltd.

Interested in learning more? Please take a look at our short validation of the Psilocybin-QTest


Validation by authorities - State-regulated drug checking

The QTest where officially validated & approved for the federal drug-checking program ALIVE in the federal state of Thuringa (Germany). They have already been used on-site for 3 years and form the backbone of the mobile analysis methods. An evaluation of mobile drug checking is currently being prepared for publication by the Berlin Charité.


Confirmation from others

The functionality and the precision of our QTests have already been tested & validated by a lot of customers and third parties:


Psilocybe Expert & Shroomery Community founder Alan Rockefeller is a convinced user of our QTests, straight from the beginning. He uses the Psilo-QTests to quantify the psilocybin in rare species like in the YT Series "crime pays but botany doesn't"


Doma aka Magic Myco, has extensively analyzed our tests in parallel to his HPLC analysis and pure standards, as well as already evaluating our tests with spectrophotometric methods:



Psychedelic Passage compared the results of our rapid tests with laboratory tests from Energy Control International (a long-standing drug-checking program in Spain) for identical samples and come to convincing results

  • Psilocybin Q-Test Result: According to our at-home test, there is approximately 1.1% psilocybin and psilocin combined in a 1-gram sample of our mushrooms
  • Psilocybin HPLC Test Result: The lab analysis revealed a psilocybin content of 1.08% psilocybin and psilocin combined
  • complete Psychedelic Passage QTest review

"The results from the two tests are very close. The slight difference between the at-home test prediction and the lab test result is negligible. Considering that both methods can fluctuate within 10% in accuracy, the minor variation is well within the acceptable range of variation. This indicates that Miraculix QTests are reliable tools for assessing the potency of your psilocybin mushrooms."

Important Disclaimer


This test is not suitable for identifying Psilocybin/Psilocin or psilocybin-containing mushrooms. For example: When picking wild mushrooms, there is a risk of confusing or mixing them with the toxic Galerina Maginata, which grows in similar habitats. The QTest does not test for fungal toxins, so it cannot be used for mushroom identification. However for test psilocybin-containing mushrooms, the QTest quantifies the amount of the active substance present.


Psilocybin is not uniformly distributed within mushrooms! Even mushrooms from the same batch can vary significantly in their psilocybin content. To ensure accuracy, it is essential to homogenize the mushroom material thoroughly by using a grinder, sharp knife, or cutting board before conducting the test. The test result will indicate the percentage of psilocybin per gram in the homogenized material.  The total measurement will be presented in terms of psilocybin, encompassing both psilocybin and psilocin.



Testing a sample with our test kits should not be considered a guarantee of safety. Essential risks are associated with the consumption of any substance. Various factors can influence testing outcomes and reactions. Miraculix does not take responsibility for the interpretation of test result. Sample responses can differ due to lighting variations and specific substance blends being tested. The use or misuse of this information is not the responsibility of miraculix.



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  1. Lennard

    used this test for my homegrown mushrooms. Instructions were well described in the manual. I had a question for the evaluation and the team responded quikcly. Will definitely order again!

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